Journal: Angiogenesis
Article Title: BMP10 functions independently from BMP9 for the development of a proper arteriovenous network.
doi: 10.1007/s10456-022-09859-0
Figure Lengend Snippet: Fig. 8 BMP10, but not BMP9, suppresses the development of AVMs caused by ENG- deficiency. a–e CD31 and SMA immunofluorescence staining on retinas isolated from P7 control (PBS-treated Scl-CreER-negative Eng-iKO, a, n = 28 retinas), PBS-treated Scl-CreER;Eng-iKO (b, n = 16), BMP9-treated Scl-CreER;Eng- iKO (c, n = 8), BMP10-treated Scl-CreER;Eng-iKO (d, n = 8), and BMP9/BMP10-treated Scl-CreER;Eng-iKO (e, n = 10) mice. Arrows mark arterio- venous shunts. a artery, v vein. Scale bars, 500 μm. f Quantifi- cation of the number of AVMs. Data are mean ± SD. One-way ANOVA followed by Tukey’s post hoc test
Article Snippet: Control PBS, 100 ng of mouse BMP9 protein (R&D systems, 5566-BP), or 100 ng of mouse BMP10 protein (R&D systems, 6038-BP) was injected intraperitoneally and daily on the opposite side of the milk spot until the sample collection.
Techniques: Immunofluorescence, Staining, Isolation, Control